First Semester

The purpose of this course is to acquaint students with the common experimental

methods used in biotechnology. The course should impart students with skills to be able

to work in research and development.

Expected Learning Outcomes

Upon successfully completing this course students should be able to:

1. Describe, classify and characterize the various physicochemical analysis methods.

2. List key advantages and limitations of advanced analytical methods.

3. Select and rationalize selection of analytical methods and data processing protocols for

given biological samples.

Course Content

Analytical methods employed in isolation, purification, identification and quantitative

estimation of organic and inorganic compounds; Principles and application of

instrumental analysis; Analysis of samples for organic and inorganic substances;

Sampling, sample preparation and analysis; Chromatography (HPLC, gas and liquid

chromatography), Spectroscopy (NMR, IR, MS), Thermal analysis including PCR,

Electrophoresis etc. X-ray diffraction, electron microscopy, radiography.

Mode of Delivery

Lectures, discussions; analysis of problematic exercises and practical laboratory work

Instructional Material and /or equipment

Course notes, handouts, practical manual, computer, LCD projector, analytical chemistry

equipment, reagents and other required materials for practical work.

Course Assessment

Continuous Assessment Tests 15%

Assignments and practicals 15%

End of semester examination 70%

Total 100%

Core Reading Materials for the Course

1. Wilson, K. & Walker, J. (Eds.). (2000). Principles and Techniques of Biochemistry. 5th edition. Cambridge University Press. ISBN-10: 052165873X, ISBN-13:

9780521658737.

2. Skoog, D. A., Holler, F. J. & Crouch, S. R. (2006). Principles of Instrumental Analysis,

6th edition. Brooks Cole Publishers, ISBN-10: 0495012017, ISBN-13: 978-0495012016

Recommended Reference Materials

1. Carmona, P., Navarro, R. & Hernanz, A. (Eds.). (1997). Spectroscopy of Biological

Molecules: Modern Trends. 1st edition. Kluwer Academic Publishers. ASIN:

B00Y34PVIU or Springer ISBN-10: 9401063699, ISBN-13: 978-9401063692

2. Valeur, B. & Berberan-Santos, M. N. (2013). Molecular Fluorescence: Principles and

Application. 2nd Edition. Wiley -VCH. ISBN-10: 3527328467, ISBN-13: 978-3527328468

3. Krishna, N. R. & Berliner, L. J. (Eds.). (2003). Protein NMR for the Millennium

(Biological Magnetic Resonance). Springer 2002 edition. ASIN: B000WEFYK0

4. Selected reading at http://pubs.acs.org/